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Gleditsia sinensis is widely used as a medicinal plant in Asia, especially in China. Triterpenes, alkaloids, and sterols were isolated from Gleditsia species. Among them, triterpenoid saponins are very important metabolites owing to their various pharmacological activities. However, the triterpenoid saponin biosynthesis pathway has not been well characterized. In the present study, we performed de...
Key messageCYP716A179, a cytochrome P450 monooxygenase expressed predominantly in tissue-cultured stolons of licorice (Glycyrrhiza uralensis), functions as a triterpene C-28 oxidase in the biosynthesis of oleanolic acid and betulinic acid. Abstract Cytochrome P450 monooxygenases (P450s) play key roles in the structural diversification of plant triterpenoids. Among these, the CYP716A subfamily, which...
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