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The objectives of this study were to develop an amino acid test diet for Pacific threadfin (Polydactylus sexfilis) and estimate its lysine (Lys) requirement based on growth performance. A basal test diet was formulated using Pacific threadfin muscle and a mixture of precoated crystalline amino acids as the protein sources to achieve the same amino acid profile as in Pacific threadfin muscle with the...
. Four semi-purified diets, containing crystalline amino acids (CAAs), were fed to juvenile red sea bream, Pagrus major in order to ascertain the ideal dietary amino acid pattern for this species. A control diet containing 50% casein–gelatin as protein sources, but no CAAs were fed to the fish. The other diets contained 30% casein–gelatin and 20% CAAs. CAAs were added to diets to simulate with amino...
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