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Objectives To determine the effects of the Sus scrofa matrix attachment region (SusMAR) on transgene expression in HEK293T cells. Results Three expression vectors with the MAR at different sites in the PiggyBac (PB) transposon vector backbone were compared: two MARs flanking the β-galactosidase (β-gal) expression cassette, and one at the upstream or downstream site. Bos taurus MAR (BosMAR) and a...
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