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Real time monitoring is critical to apply continuous bioprocesses successfully. This requires the integration of flow cells into continuous reactors. Potential continuous steps include protein precipitation, used in plasma purification, antibody enrichment and virus inactivation. Consequently we developed real-time monitoring of protein precipitation in a tubular reactor by pH, conductivity, turbidity...
Heterologous proteins are often poorly expressed in Escherichia coli and especially small peptides are prone to degradation. N pro autoprotease fusion proteins, deposited as inclusion bodies in E. coli, are a versatile tool for peptide and protein overexpression and generate an authentic N terminus at the target molecule. Autoproteolytic cleavage and subsequent release of the fusion partner...
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