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The cell surface of Schizosaccharomyces pombe is negatively charged due to the presence of pyruvylated oligosaccharides, which is important for cell–cell recognition. However, the mechanism of pyruvate supply to oligosaccharides is not clearly understood. Here, we analyzed three putative phosphoenolpyruvate (PEP) transporter genes (pet1+, pet2+, and pet3+) in S. pombe, identified by sequence homology...
Fission yeast flocculates nonsexually by induction of the flocculin encoded by gsf2+ which is controlled by the positive regulator Mbx2. Here, we report a novel gene designated gsf1+ found to be a negative regulator of nonsexual flocculation. We identified gsf1+ as a multicopy suppressor of a sam2 mutation, which caused growth sensitivity to Ca2+ and also found a nonsense mutation in gsf1+ in a previously...
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