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The optimization of a chromatographic process using immobilized metal affinity chromatography requires an understanding of the factors that govern the interaction between proteins and immobilized metal ions. Factors, such as concentrations of protein, NaCl and imidazole were investigated to elucidate kinetics of adsorption of alcohol dehydrogenase (ADH) onto a dye-iminodiacetic acid matrix (dye-IDA...
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