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Single nucleotide polymorphisms (SNPs) are the most common genetic variations that can be genotyped effectively by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Although PCR-RFLP has become the popular technique, search for available restriction enzymes and design of feasible primers for SNP genotyping is still a challenging task. An available restriction enzyme at...
In this paper, a method named chaotic inertia weight particle swarm optimization (CIWPSO) for designing polymerase chain reaction experiments with confronting two-pair primers (PCR-CTPP) is proposed. PCR-CTPP is a time-and cost-effective technique for SNP (single nucleotide polymorphism) genotyping. In the past decades, many SNPs were genotyped successfully by it. However, computation of feasible...
Many primer design methods have been proposed to provide feasible primer sets for performing polymerase chain reaction (PCR) experiments. However, the majority of these methods require a long time to obtain a feasible solution since large quantities of DNA template need to be analyzed and a specific PCR product size is not provided. Recently, particle swarm optimization (PSO) has been applied to solve...
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