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The fermentation broth of Bacillus cereus NTU-FC-4 was precipitated with 70% acetone to obtain crude enzyme. Chitosanases in the crude enzyme were then extracted by reversed micelles. It was found that proper amount of crude enzyme should be first dissolved in the 50.0mM phosphate buffer containing 96.0mM sodium chloride to make a 1.0mg/ml protein solution. After adjusting the pH of the crude enzyme...
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