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Laser scanning confocal technology was used to study relationship between apoptosis and changes of Ca2+ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effects of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation on HepG-2 cells was completed by fluorescence microscope. Apoptosis of HepG-2 cells...
The aim of this paper is to investigate the pro-apoptosis effect of isothiocyanates (ITCS) on human gastric adenoma cells SGC-7901 and its mechanism. SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential...
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+]i in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with TMRE and Fluo-3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]i in the cells are observed using LCSM. The results of double staining with TMRE and...
This study analyzed the effect of Asparagus polysaccharides on the migration time of erythrocytes in tumor- bearing organisms. A mouse tumor model was developed in which mice were intraperitoneally injected with high, medium, and low dosages of Asparagus polysaccharides for 7 d. The erythrocytes were collected and prepared into suspensions, and the migration time of cells were measured using high...
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca24]; in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with TMRE and Fluo- 3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]; in the cells are observed using LCSM.The results of double staining with TMRE and...
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