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This review compiles and discusses previous reports on the identity of wall-associated enzymes (WAEs) in fungi and addresses critically the widely different terminologies used in the literature to specify the type of bonding of WAEs to other entities of the cell wall compartment, the extracellular matrix (ECM). A facile and rapid fractionation protocol for catalytically active WAEs is presented, which...
This review provides biochemical, analytical, and biological background information relating to β-N-acetylhexosaminidase (HexNAc'ase; EC 3.2.1.52) as an emerging target for the design of low-molecular-weight antifungals. The article includes the following: (1) a biochemical description of HexNAc'ase (reaction catalyzed, nomenclature, and mechanism of action) that sets it apart from other, similar...
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