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The nucleotide sequence (2617 nucleotides) of virus-like double-stranded (ds) RNA 3a in a diseased isolate, Log1/3-8d2(Ld), of the ascomycete fungusOphiostoma novo-ulmihas been determined. One strand of the dsRNA contains an open reading frame (ORF) with the potential to encode a protein of 718 amino acids, and the complementary strand contains two smaller ORFs with the potential to encode proteins...
The nucleotide sequences of 2 of the 10 mitochondrial double-stranded (ds) RNA segments in a diseased isolate, Log1/3-8d 2 (Ld), ofOphiostoma novo-ulmi,RNA-7 (1057 nucleotides) and RNA-10 (317–330 nucleotides), have been determined. Both RNAs are A-U-rich, but in Southern and Northern blots, no hybridization with mitochondrial DNA or RNA could be detected. Only very short open reading frames...
We have recently described a novel strategy for engineering resistance to African cassava mosaic virus (ACMV) in transgenicNicotiana benthamianaplants using a virus-inducible promoter to control the expression of a plant ribosome-inactivating protein (RIP) transgene (Y. Honget al., Virology220, 119–127, 1996). Here, we have used a potato virus X (PVX) vector to express the ACMV transactivator protein,...
Ribosome-inactivating proteins (RIPs) are naturally occurring plant toxins that exhibit antiviral activity against a diverse range of plant and animal viruses. Here, the action of dianthin, a potent RIP isolated fromDianthus caryophyllus,has been exploited to engineer resistance to a plant DNA virus, African cassava mosaic virus (ACMV), in transgenicNicotiana benthamiana.To achieve this, dianthin...
Coat protein (CP) gene of the Chinese squash leaf curl virus (SqLCV-C) was amplified through PCR, cloned and completely sequenced. Based on the comparisons at the levels of both CP gene nucleotide and CP-deduced amino acid sequences with other geminiviruses, SqLCV-C is confirmed to be distinct from the American squash leaf curl virus (SqLCV-E). It is a new geminivirus transmitted by whitefly Bemisia...
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