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We searched for sex markers in mature male and female Takifugu rubripes gonads and tailfins by cDNA-amplified fragment length polymorphism (AFLP) performed on pooled cDNA samples. Five sex markers (TDF1-5) were identified, cloned, characterized and confirmed on individual samples of cDNA and genomic DNA, showing good agreement with phenotypic sex. Among them, four markers (TDF1-4) derived from gonads...
By screening the whole genome sequence of Takifugu rubripes available in DDBJ, a total of 49647 simple sequence repeats (SSRs) were identified. One SSR was found every 7351 bp and the total length of all SSRs accounts for 0.77% of the whole genome, about 365 Mb in length. The most frequent six SSR types are di-, tetra-, tri-, penta-, hexa- and mononucleotide types, accounting for 71.30%, 13.77%, 7...
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