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In these studies, [ 3 H]epibatidine is used as the radioligand to characterize recombinant bovine α3β4 nicotinic acetylcholine receptors (nAChRs) expressed in HEK 293 cells. Specific binding reaches equilibrium quickly and is saturable with a K d value of 0.66 nM. The affinities of the several cholinergic agents were determined, including nicotine (K i , 0.5 μM), cytisine (K...
In these studies, [ 3 H]epibatidine is used as the radioligand to characterize nicotinic acetylcholine receptors (nAChRs) from bovine adrenal medulla. Specific binding reaches equilibrium within 30 min, and is saturable with a K d value of 0.5 nM. The affinities of several cholinergic agents were determined, including nicotine (K i , 0.2 μM), cytisine (K i , 0.4 μM),...
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