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Poly(A) + RNA isolated from the livers of normal rats and of rats suffering from an acute inflammation was translated in a cell-free translation system from rabbit reticulocytes. The translation products were immunoprecipitated with specific antisera against α1-acid glycoprotein, α2-macroglobulin, transferrin, α1-proteinase inhibitor and albumin. 15 to 21 h after intramuscular injection of...
The biosynthesis and secretion of α2-macroglobulin was studied in rat hepatocytes primary cultures. After immunoprecipitation of α2-macroglobulin from a cell homogenate and the hepatocyte medium, two forms of α2-macroglobulin with app. M r of 176000 and 182000, respectively, were identified. A precursor-product relationship for the two α2-macroglobulin forms was demonstrated by a pulse-chase...
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