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A signal amplificatory electrochemical immunoassay with biotin–streptavidin conjunction and multienzymatic-based substrate recycling was developed in this work. Biotinylated secondary antibody (bio-IgG) was preliminarily assembled onto the immunosensor interface based on the sandwich format. Streptavidin was then loaded based on biotin–streptavidin conjunction. Owing to four identical binding sites...
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