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A β-glucosidase Bgl1A variant (A24S/F297Y) with improved ethanol tolerance was obtained by semi-rational engineering. At 30–40°C, IC50 values (the amount required for inhibiting 50% enzyme activity) of the variant for ethanol were 17–30% (v/v), 1.4- to 2.4-fold of Bgl1A. When incubating in 15% (v/v) ethanol at 30°C, the half-life of A24S/F297Y was 13min; whereas Bgl1A lost all enzyme activity within...
Objectives To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source. Results Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml−1 within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium. A novel isoenzyme, named Tvlac, was identified,...
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