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Under global climate change, alfalfa (Medicago sativa L.) development is facing a large challenge of drought, which made it crucial and necessary to improve its drought tolerance through biotechnological approaches. In alfalfa, the MsSIK1 gene was identified to be one of the water use efficiency (WUE)-related genes due to its strong expression in response to dehydration. In this study, after the 26-day-long...
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