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A sensitive and reliable reversed-phase liquid chromatography (RP-LC) with ultraviolet (UV) detection has been developed and validated for the quantification of Icariside II in rat plasma and tissues using Fermononetin as the internal standard. Protein precipitation and liquid–liquid extraction were utilized for plasma and tissue sample preparation, respectively. The analysis was successfully carried...
The aim of the study was to develop a procedure of purifying rabbit anti-rat TRβΔ polyclonal antibody from the antiserum. TRβΔ was a newly reported thyroid hormone receptor beta isoform. To gain better understanding of this new receptor, such as its tissue distribution, its specific antibody was needed. In this study, chemically synthesized peptide, which is uniquely existed in the TRβΔ, was used...
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