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Free volume variations of Nylon12/Polyvinyl alcohol (Nylon12/PVA) blends treated in supercritical carbon dioxide (ScCO₂) were investigated using positron annihilation lifetime spectroscopy. It is found that o-Ps lifetime τ₃ and the corresponding intensity I₃ in Nylon12/PVA decrease with increase of the PVA content. Being treated in ScCO₂ at 50°C and 20 MPa for 1 h, the free volume holes in Nylon12/PVA...
His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length...
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