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A surface-enhanced Raman signal at about 225 cm -1 was detected in oxygen-evolving photosystem II (PS II) membranes treated with 1 M CaCl 2 (which removes three extrinsic proteins but does not extract Mn). The signal is not seen in Tris-treated preparations (which retain none of the three proteins nor Mn) nor in NaCl-treated preparations (which retain Mn and the 33-kDa extrinsic...
Fluorescent emission kinetics of isolated spinach chloroplasts have been observed at room temperature with an instrument resolution time of 10 ps using a frequency doubled, mode-locked Nd:glass laser and an optical Kerr gate. At 685 nm two maxima are apparent in the time dependency of the fluorescence; the first occurs at 15 ps and the second at 90 ps after the flash. The intervening minimum occurs...
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