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Trehalose‐6‐phosphate hydrolase (TreA) belongs to glycoside hydrolase family 13 (GH13) and catalyzes the hydrolysis of trehalose 6‐phosphate (T6P) to yield glucose and glucose 6‐phosphate. The products of this reaction can be further metabolized by the energy‐generating glycolytic pathway. Here, crystal structures of Bacillus licheniformis TreA (BlTreA) and its R201Q mutant complexed with p‐nitrophenyl‐α‐d‐glucopyranoside...
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