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β-Mannanase (EC 3.2.1.78) is a key enzyme to hydrolyze the β-mannosidic linkages in mannan and heteromannan. The expression of a wild type β-mannanase (manWT) of Aspergillus sulphureus in Pichia pastoris is not high enough for its application in feed supplement. To earn a high expression level, the manWT gene was firstly optimized to manM according to the code bias of P. pastoris, which was then...
Using RT-PCR and rapid amplication of cDNA ends (RACE) techniques, a 1345bp full-length cDNA fragment was obtained from Aspergillus sulphureus. The gene, designated MANN, codes for a 383-residue with a calculated mass of 41,389Da. MANN displayed amino acid sequence similarity to the β-mannanase of Aspergillus aculeatus and Trichoderma reesei, members of the glycoside hydrolase family 5. The recombinant...
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