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A gold nanorod‐locked nucleic acid nanobiosensor for dynamic single‐cell gene expression analysis in living cells and tissues is developed. The nanoparticle facilitates endocytic delivery and dynamic monitoring of the gene expression in human umbilical cord endothelial cells, mouse skin tissues, mouse retina tissues, and mouse cornea tissues at the single‐cell level.
On page 6034, P. K. Wong and co‐workers demonstrate a nanorod‐based biosensor for dynamic single‐cell analysis in native tissue microenvironments. The biosensor is capable of monitoring spatiotemporal mRNA expression in primary human cells, capillary networks, and animal tissues, including the skin, retina, and cornea, challenged mechanically and biochemically.
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