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The monoethyl esters of the long chain fatty acids (biodiesel) were prepared by alcoholysis of Jatropha oil, a non-edible oil, by a lipase. The process optimization consisted of (a) screening of various commercial lipase preparations, (b) pH tuning, (c) immobilization, (d) varying water content in the reaction media, (e) varying amount of enzyme used, and (f) varying temperature of the reaction. The...
Addition of bovine serum albumin (BSA) as a proteic feeder facilitates obtaining cross-linked enzyme aggregates (CLEAs) in cases where the protein concentration in the enzyme preparation is low and/or the enzyme activity is vulnerable to the high concentration of glutaraldehyde required to obtain aggregates. CLEAs of Pseudomonas cepacia lipase and penicillin acylase were prepared. CLEA of lipase prepared...
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