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The half lives of polyphenol oxidase and trypsin at 50% (v/v) co-solvent (water miscible) were from 23 to 176 h when polarity index of the co-solvent was >5.8 or if the co-solvent was an alcohol. Polyphenol oxidase, trypsin, chymotrypsin and β-glucosidase lost only 10% activity in such water/organic co-solvent mixtures when the organic co-solvents were present in the concentration ranges of 55–90%,...
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