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In this work, a streaming potential analyzer was constructed based on a composite PDMS-on-glass microchannel and was applied to the label-free DNA detection. Peptide nucleic acid (PNA) probes were covalently attached to the glass substrate to facilitate hybridization with the target DNA at low ionic strength and provide minimized baseline signal for streaming potential analysis. The hybridization...
This paper describes a microwell-based microdevice for performing quantitative bioanalysis. This microdevice combined the passive pumping by degassed polydimethylsiloxane (PDMS) with serial operations including solution dispensing, plates splitting and plates stacking. We name this microdevice “stacking chip”. To use the stacking chip in quantitative bioanalysis, nanoliter solutions were first dispensed...
In this paper we study enzymatic and heat profile modeling for Thermosequencing [1-5], and discuss the real time 2D and 3D finite element simulation results for chemical concentrations involved in the detection system. The modeling simulates not only the heat released through the DNA polymerization reaction and its corresponding temperature change, but also the heat and sensing efficiencies of different...
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