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Three anticoagulants combining large peptide recombinant hirudin variants (rHV2-K47) and Arg–Gly–Asp (RGD) motif related to platelet aggregation were generated, i.e. sequences CRFPRGDADPYCE and CNPRGDFRCI were added to the C-terminus of hirudin to obtain RGD-hirudin 1 and 2, respectively, and the sequence RGDSE was inserted between residues 53–54 of hirudin to obtain RGD-hirudin 3. All products exhibited...
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