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The acquisition of DNA and the loss of genetic information are two important mechanisms that contribute to strain-specific differences in genome content. In this study, comparative genomics has allowed us to infer the roles of genomic rearrangement and changes in both distribution and copy number of the insertion element, IS1096, in the evolution of Mycobacterium smegmatis mc 2 155 from its...
The genome of Mycobacterium smegmatis mc 2 155 contains a 56kb duplicated region. We isolated a mutant of mc 2 155 lacking this duplication (ΔDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of ΔDRKIN for the study of genes contained within the duplicated region.
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