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We report here on an improved, completely defined culture system for producing embryos in vitro which mimics development in vivo. This system avoids the confounding effects of the many unknowns introduced by the multivariate components of the serum or by unknowns attached to bovine serum albumin (BSA). Zygotes were obtained from superovulated rabbits and cultured in modified defined RPMI 1640:Dulbecco's...
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