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In this paper, a multilayer elastomeric microfluidic device with micro cell sieves was developed for an assay of drug induced cytotoxicity. We evaluated the cytotoxic effects of anti-cancer drugs tamoxifen (TAM) and camptothecin using MDA-MB-231 breast cancer cells and human chronic myeloid leukaemia K562 cells respectively. To validate cell morphology and viability on the microfluidic chip, fluorescent...
This paper presents an efficient search algorithm utilizes the relationship between local motion similarity and the variation degree of motion vector. The main feature of proposed algorithm are: the motion vectors are classified as the unchanged, the slightly changed and the greatly changed motion vector according to corresponding local motion similarity; then different search schemes are adopted...
An effective technique to concentrate the lipid-coated microbubbles within a droplet based on the surface acoustic wave is demonstrated. A droplet was partially placed on a surface acoustic wave propagation path, and microbubbles were concentrated at the center of the droplet due to the asymmetry. The device consist a lithium niobate piezoelectric substrate and a pair of interdigital transducer electrodes...
Microbubbles coated by surfactant or polymer have been shown to be the most effective contrast agents for diagnostic ultrasound contrast imaging and targeted molecular imaging probes. The microbubbles produced by flow-focusing units have a narrower size distribution than that of the traditional methods such as sonication or agitation and is nearly monodisperse in size distribution, but with very limited...
Microbubbles promise not only as effective acoustic probes for ultrasonic molecular imaging,but also as drug carrier vehicles by conjuncting to specific antibody or ligand. Microbubble's targeting efficiency to certain cells or moleculars is one of the major challenges of being as the acoustic probes. In this study, we developed tumor targeted contrast microbubbles by binding LyP-1 (a cyclic nonapeptide...
Using ultrasonic contrast microbubbles as acoustic biomarkers and drug carrier vehicles by conjugating tumor specific antibody to microbubbles has shown great potential in ultrasonic tumor molecular imaging or drug-delivery and therapy. Microbubble probe targeting efficiency is one of the major challenges. In this study, we developed a novel method to evaluate the targeting capability and efficiency...
A microfluidic array with micro cell sieves was designed to fabricate multiple cells array for high-throughput cell cytotoxicity screening. Micro channels in both directions (column and row) were individually addressable by using a set of elastomeric monolithic array valves, enabling parallel loading of various cell lines in one direction and introducing of different toxins in the other direction...
A microfluidic array with micro cell sieves was designed to fabricate multiple cells array for high-throughput cell cytotoxicity screening. Micro channels in both directions (column and row) were individually addressable by using a set of elastomeric monolithic array valves, enabling parallel loading of various cell lines in one direction and introducing of different toxins in the other direction...
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