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To explore a new method of in vitro culture and purification of rat corpus cavernosum endothelial cells (CCECs). Male Sprague‐Dawley rats' penile tissue were digested with elastase or collagenase combined with mechanical extrusion to isolate and culture the CCECs. The fixed‐point digestion method was used to purify the primary cells. High‐purity CCECs were successfully isolated. Following the digestion...