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A highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (Apt-GNPs) for amplification. Human α-thrombin, an important physiological protease found in blood, was chosen as the target protein. Captured by immobilized aptamers, thrombin was determined on-line using Apt-GNPs to enhance both frequency...
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