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The submerged cultivation of Pleurotus ostreatus var. florida on wheat gluten led to an increased solubility of the substrate. The coding sequence of one of the extracellular peptidases was obtained by PCR with degenerated primers and RACE. The sequence amounted to 1161bp, translating into 386 aa with a molecular mass of 38.7kDa. By comparison with homologous proteins the new enzyme was classified...
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