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To investigate the relationship between the toxicity of puffer fish and the distribution of tetrodotoxin-producing bacteria in puffer fish Fugu rubripes collected from the Bohai Sea of China, bacteria were isolated from each organ (ovaries, livers, intestines and gallbladders) and screened for tetrodotoxin (TTX) production. 20 out of 36 isolated strains were found to produce TTX in vitro. In the organs...
Three puffer fishes, Fugu rubripes, collected from the Bohai Sea of China were examined for tetrodotoxin-producing microorganisms. An actinomycete isolated from the ovaries of the puffer fishes was found to produce tetrodotoxin. After being cultured at 28°C for 7 days, cells were harvested by centrifuge and disrupted by ultrasonication. The toxin was purified from the cell lyzate by ultrafiltration,...
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