Search results for: Mohammad Ali Khalili

Items from 1 to 20 out of 38 results

article

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

Fatemeh Dehghanpour, Mohammad Ali khalili, Esmat Mangoli, Ali Reza Talebi, more

Sperm processing for assisted reproductive technologies (ART) aims to separate immotile and debris from the motile spermatozoa in the semen. The purpose of this study was to assess the effect of free centrifuge sorting (FCS) approach based on a combination of rheotaxis and swim‐up on sperm biological characteristics and ICSI clinical outcomes. Each semen sample was splitted into two equal parts for...

article

The detrimental effect of cell phone radiation on sperm biological characteristics in normozoospermic

Mohammadmehdi Hassanzadeh‐Taheri, Mohammad Ali Khalili, Ali Hosseininejad Mohebati, Mahmood Zardast, more

Andrologia > 54 > 1 > n/a - n/a

Radiofrequency electromagnetic radiation emitted from cell phone has harmful effects on some organs of the body, such as the brain, heart, and testes. This study aimed to assess the effects of cell phones on sperm parameters, DNA fragmentation, and apoptosis in normozoospermic. Normal sperm samples were divided into two groups of control and case. The samples from the case were placed for 60 min at...

article

Proliferating cell nuclear antigen presentation, as a marker of folliculogenesis, in the transplanted ovarian tissue

Mahboubeh Vatanparast, Mojgan Karimi Zarchi, Ali Nabi, Mohammad Ali Khalili

Journal of Obstetrics and Gynaecology Research > 47 > 12 > 4340 - 4349

Aim One of the most important ways to understand the ovarian biology is studding the initiation of primordial follicle development and subsequent folliculogenesis control. In this study, proliferating cell nuclear antigen (PCNA) presentation was used as a marker of follicular development in the thawed ovarian tissue (OT) following transplantation onto chick embryo chorioallantoic membrane (CAM) using...

article

Use of zona pellucida‐bound spermatozoa as a natural selection in improvement of ICSI outcomes: A systematic review and meta‐analysis

Mahin Izadi, Mohammad Ali Khalili, Amin Salehi‐Abargouei, Mohammad Ebrahim Rezvani, more

Andrologia > 53 > 6 > n/a - n/a

Zona pellucida (ZP)‐bound spermatozoa have normal morphology and motility and can enhance the ICSI outcomes. Selection of zona pellucida‐bound spermatozoa is recently considered to find functional spermatozoa for ICSI. This study reviewed the efficacy of ZP‐bound sperm selection on the ICSI outcomes includes fertilisation rate, embryo quality, embryo transfer rate and clinical pregnancy rate. The...

article

Impact of biological and artificial seminal fluids on sperm parameters and DNA status in asthenozoospermic ejaculates

Lida Gholizadeh, Azam Agha‐Rahimi, Saeed Ghasemi‐Esmailabad, Behnam Maleki, more

Andrologia > 53 > 5 > n/a - n/a

The chemical composition and physiological properties of seminal fluid (SF) affect sperm quality. The objective was to investigate the effects of in vitro exposure of artificial seminal fluid (ASF) and biological seminal fluid (SF) on sperm quality. Asthenozoospermic ejaculates (n = 20) were divided into two aliquots. The first aliquot was centrifuged for obtaining asthenozoospermic SF. The second...

article

Does in vitro application of pentoxifylline have beneficial effects in assisted male reproduction?

Maryam Mahaldashtian, Mohammad Ali Khalili, Stefania Annarita Nottola, Bryan Woodward, more

Andrologia > 53 > 1 > n/a - n/a

Application of nonspecific phosphodiesterases inhibitors, such as pentoxifylline (PTX), is a strategy utilised to aid sperm selection from immotile sperm samples prior to ICSI. No extensive studies have yet been performed to verify the safety of the clinical outcomes of ICSI after PTX administration. In this article, we summarise the data reported in the literature that assess the implication of in...

article

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

Fatemeh Anbari, Mohammad Ali Khalili, Azam Agha‐Rahimi, Behnam Maleki, more

Andrologia > 52 > 11 > n/a - n/a

Evaluation of sperm integrity may predict the in vitro fertilisation (IVF) outcomes. The aim was to evaluate the relationship between the sperm DNA fragmentation (sDNAf) with embryo morphology and morphokinetic using time‐laps monitoring (TLM) and to select the best time points for normalisation in IVF setting. After evaluating the fertilisation and pronuclei (Z) scoring, 328 normally fertilised oocytes...

article

Effective dosage of growth differentiation factor‐9β in folliculogenesis and angiogenesis in the sheep ovarian tissues grafted onto chick embryo chorioallantoic membrane

Mahboubeh Vatanparast, Mojgan Moshrefi, Nahid Yari, Mohammad Ali Khalili, more

Journal of Obstetrics and Gynaecology Research > 46 > 8 > 1384 - 1392

Aim Scientists have tried to culture and transplant the ovarian tissues (OT), to preserve fertility in cancer patients. However, one of the main limitations to the applicability of this technique is the folliculogenesis disruption after transplantation. Due to the roles exerted by growth differentiation factor‐9β (GDF9β), we decided to determine the most effective dose of GDF9β on promotion of folliculogenesis...

article

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

Esmat Mangoli, Mohammad Ali Khalili, Ali Reza Talebi, Azam Agha‐Rahimi, more

Andrologia > 51 > 8 > n/a - n/a

Our objective was to evaluate the effect of IMSI on embryo kinetics and clinical outcomes in patients with different aetiologies of male infertility. A total of 150 couples with different aetiologies of male infertility were randomly divided into ICSI and IMSI treatment groups (n = 75). ICSI was done accordingly. For IMSI group, the sperm selection was done using MSOME criteria and then injected....

article

Is there any correlation between apoptotic genes expression in cumulus cells with embryo morphokinetics?

Azita Faramarzi, Mohammad Ali Khalili, Masoumeh Golestan Jahromi

Molecular Biology Reports > 2019 > 46 > 4 > 3663-3670

The aim was to assess the correlation between apoptotic genes of cumulus cells (CCs) with embryo morphokinetics as non invasive methods for embryo selection. Evaluation of cleavage activity among in vitro-fertilized embryos was dependent on determining not only expression profiles of pro- and anti-apoptotic genes in CCs surrounding ovulated oocytes but also morphokinetic parameters such as time of...

article

Medium containing different concentrations of catalase as a strategy for optimising sperm parameters and chromatin in normospermic persons

Masoomeh Mohammadzadeh, Vahid Ramazani, Mohammad Ali Khalili, Hamed Hamishekar, more

Andrologia > 51 > 4 > n/a - n/a

The aim of this study was to comprise the effect of catalase on sperm parameters and chromatin in normospermic persons. Semen samples were obtained from fertile men. A certain amount of different concentrations of catalase (0.1, 1, 10, 50, 100, 150 and 200 IU.ml) was added to each vial containing semen. Control group had similar condition to treated groups without treatment. Treatment was done for...

article

GDF9-β promotes folliculogenesis in sheep ovarian transplantation onto the chick embryo chorioallantoic membrane (CAM) in cryopreservation programs

Mahboubeh Vatanparast, Mohammad Ali Khalili, Nahid Yari, Bryan Woodward, more

Archives of Gynecology and Obstetrics > 2018 > 298 > 3 > 607-615

Purpose Ovarian tissue (OT) cryopreservation is a treatment option for fertility preservation among young cancer patients. However, the procedure may involve a reduction in the GDF9-β expression and a delay in follicular growth after thawing and transplantation. The aim of this study was to evaluate whether supplementation of GDF9-β can compensate the reduction of this factor during the cryopresevation...

article

Cosmetic micromanipulation of vitrified-warmed cleavage stage embryos does not improve ART outcomes: An ultrastructural study of fragments

Somayyeh Safari, Mohammad Ali Khalili, Zeinab Barekati, Iman Halvaei, more

Reproductive Biology > 2017 > 17 > 3 > 210-217

The aim was to study the ultrastructure of cytoplasmic fragments along with the effect of cosmetic micromanipulation (CM) on the morphology and development of vitrified-warmed embryos as well as assisted reproductive technology (ART) outcomes. A total of 96 frozen embryo transfer (FET) cycles were included in this prospective randomized study. They were divided into three groups of CM (n=32), sham...

article

Comparing the effects of different in vitro maturation media on IVM outcomes of MI oocytes

Farzaneh Fesahat, Azita Faramarzi, Mohammad Hasan Sheikhha, Razieh Dehghani Firouzabadi, more

Middle East Fertility Society Journal > 2017 > 22 > 3 > 174-177

Optimizing in vitro maturation (IVM) media for better assisted in vitro fertilization (IVF) outcome has been the matter of interest in recent years. Commercial media for IVM have been commonly used while, they have several disadvantages, such as limited shelf life and more cost than standard culture media for IVF centers, where IVM services are not routinely performed.This study was performed to compare...

article

Vitrification of human immature oocytes before and after in vitro maturation: a review

Mohammad Ali Khalili, Abbas Shahedi, Sareh Ashourzadeh, Stefania Annarita Nottola, more

Journal of Assisted Reproduction and Genetics > 2017 > 34 > 11 > 1413-1426

The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment...

article

Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review

MARJAN OMIDI, AZITA FARAMARZI, AZAM AGHARAHIMI, MOHAMMAD ALI KHALILI

Journal of Microscopy > 267 > 3 > 253 - 264

Optimizing the efficiency of the in vitro fertilization procedure by improving pregnancy rates and reducing the risks of multiple pregnancies simultaneously are the primary goals of the current assisted reproductive technology program. With the move to single embryo transfers, the need for more cost‐effective and noninvasive methods for embryo selection prior to transfer is paramount. These aims require...

article

No cytotoxic effects from application of pentoxifylline to spermatozoa on subsequent pre-implantation embryo development in mice

Mohammad Ali Khalili, Azam Agha-Rahimi, Fatemeh Sadeghian, Iman Halvaei

Middle East Fertility Society Journal > 2017 > 22 > 2 > 132-135

The aim was to assess the effect of spermatozoa exposed to PTX on the rates of fertilization and embryo development and apoptotic cells within blastocysts in an animal model. Mice Oocytes were inseminated with spermatozoa exposed to 3.6mmol PTX for 30min, or with neat spermatozoa. Then fertilization and embryo development rate, blastocyst formation and quality, as well as total cell number of blastocyst,...

article

Clinical outcome of assisted reproductive technologies in advanced aged men

Marjan Omidi, Iman Halvaei, Mohammad Ali Khalili, Shahin Ghazali, more

Middle East Fertility Society Journal > 2017 > 22 > 2 > 111-114

The purpose was to establish whether the advanced male age may influence the outcomes in a controlled assisted reproductive technology (ART) program.This is an experimental study.Research and Clinical Center for Infertility of Yazd.A total of 63men undergoing ART cycles.In a retrospective study, the outcome of intracytoplasmic sperm injection (ICSI) in ART cycles in two groups of patients was studied...

article

Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity

Ali Nabi, Mohammad Ali Khalili, Farzaneh Fesahat, Alireza Talebi, more

Cryobiology > 2017 > 76 > C > 59-64

The freeze–thaw process results in reduced motility, viability and fertilization potential of human spermatozoa. So, a variety of substances were evaluated in order to enhance human sperm resistance to the stress of cryopreservation, such as Pentoxifylline (PTX) for improving the Intracytoplasmic sperm injection (ICSI) outcomes. The aim was to investigate the effect of PTX on sperm parameters and...

article

Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?

Azita Faramarzi, Mohammad Ali Khalili, Azam Agha-Rahimi, Marjan Omidi

Archives of Gynecology and Obstetrics > 2017 > 295 > 6 > 1515-1522

Purpose The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting. Methods Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion...

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Search results for: Mohammad Ali Khalili

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

The detrimental effect of cell phone radiation on sperm biological characteristics in normozoospermic

Proliferating cell nuclear antigen presentation, as a marker of folliculogenesis, in the transplanted ovarian tissue

Use of zona pellucida‐bound spermatozoa as a natural selection in improvement of ICSI outcomes: A systematic review and meta‐analysis

Impact of biological and artificial seminal fluids on sperm parameters and DNA status in asthenozoospermic ejaculates

Does in vitro application of pentoxifylline have beneficial effects in assisted male reproduction?

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

Effective dosage of growth differentiation factor‐9β in folliculogenesis and angiogenesis in the sheep ovarian tissues grafted onto chick embryo chorioallantoic membrane

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

Is there any correlation between apoptotic genes expression in cumulus cells with embryo morphokinetics?

Medium containing different concentrations of catalase as a strategy for optimising sperm parameters and chromatin in normospermic persons

GDF9-β promotes folliculogenesis in sheep ovarian transplantation onto the chick embryo chorioallantoic membrane (CAM) in cryopreservation programs

Cosmetic micromanipulation of vitrified-warmed cleavage stage embryos does not improve ART outcomes: An ultrastructural study of fragments

Comparing the effects of different in vitro maturation media on IVM outcomes of MI oocytes

Vitrification of human immature oocytes before and after in vitro maturation: a review

Noninvasive imaging systems for gametes and embryo selection in IVF programs: a review

No cytotoxic effects from application of pentoxifylline to spermatozoa on subsequent pre-implantation embryo development in mice

Clinical outcome of assisted reproductive technologies in advanced aged men

Pentoxifylline increase sperm motility in devitrified spermatozoa from asthenozoospermic patient without damage chromatin and DNA integrity

Is there any correlation between oocyte polarization microscopy findings with embryo time lapse monitoring in ICSI program?

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