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The authors describe three-dimensional (3D) motion pictures of dynamic micro objects obtained by parallel phase-shifting digital holographic microscope. The microscope consisted of a Mach-Zehnder interferometer, an inverted magnification optical system, a continuous wave laser, and a polarization imaging camera. An alum crystal sinking in aqueous solution of alum was set for a dynamic micro object...
Multi-modal 3D imaging based on digital holography is reviewed. In the developed system, 3D phase and 2D fluorescence distributions can be measured simultaneously. This characteristic can be used for measurement of live cell activity. We present the preliminary experimental results. One is the observation of microbeads embedded by fluorescence molecules. Second is the observation of plant cell.
Fluorescent imaging and phase contrast imaging are two major techniques for live cell studies. One can obtain those two types of images by switching illumination and filter sets, and this process usually consumes unnecessary time. In order to image dynamical cell behaviors, we have presented a multi- modal digital holographic microscopic method to obtain both quantitative phase images and fluorescent...
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