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Multi-modal 3D imaging based on digital holography is reviewed. In the developed system, 3D phase and 2D fluorescence distributions can be measured simultaneously. This characteristic can be used for measurement of live cell activity. We present the preliminary experimental results. One is the observation of microbeads embedded by fluorescence molecules. Second is the observation of plant cell.
Phase imaging and fluorescence imaging are two essential methods in biological application. A hybrid digital holographic microscopic system that enables simultaneous detection of 3D phase and 3D fluorescence images is presented. A simulation on the simultaneous imaging shows promising results compared with that of the individual detections. In the experimental results, the acquisition of 3D phase...
We succeeded in 3-D motion-picture imaging for dynamic motion of biological objects swimming at different depths by parallel phase-shifting digital holographic microscopy (PPSDHM). PPSDHM is a technique for high-quality instantaneous 3-D imaging of specimens in the microscopic field of view by single-shot recording of multiple phase-shifted holograms. We constructed a PPSDHM for recording high-speed...
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