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The isolation of DNA and RNA from the leafy gametophyte (thallus) of red algal seaweeds such as Porphyra is still a challenge because of the high content of polysaccharides. Further molecular analysis, including restriction enzyme digestion, polymerase chain reaction (PCR), and reverse transcription (RT), could be interfered by the remaining trace amount of polysaccharides. Various protocols have...
We had isolated a high molecular weight polysaccharide fraction, designated as F3, and performed a comprehensive analysis of its immunomodulatory and adjuvant activities in vivo and in vitro. In vivo, F3-treated mice showed an increase in the number of dendritic cells as well as CD4, CD8, regulatory T, B, plasma, NK, and NKT cells in the spleen. F3 also elevated the levels of multiple cytokines and...
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