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We present a microfluidic device for in-flow dielectric characterization of single biological cells. The dielectric spectrum is obtained by measuring the multiple-frequency dielectrophoresis (DEP) response of individual cells as they travel over an array of sensing and actuating electrodes. The DEP induced translation of each cell is detected by measuring the differential impedance of the array using...
We present a microfluidic device able to electroporate and measure the resultant changes in dielectric properties of single biological cells. The device can measure the dielectrophoresis (DEP) response of the electroporated cell from a few seconds after exposure to electroporating pulses. Studying electroporation at the single cell level can provide insight on the electroporation process as well as...
We present a semi-automated event identification method for collecting the dielectrophoretic signatures of cells flowing through a dieletrophoretic cytometer. The marker free dielectrophoresis (DEP) cytometer presented in this study is capable of detecting electronic signatures of cells which identifies Claussius-Mossotti factor (CMF). The CMF can in turn be used to determine properties of the cell...
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