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Lignin deposition phenomenon during the liquid-hot-water (LHW) pretreatment negatively affects substrate enzymatic digestibility (SED). To overcome this limitation of LHW, acid-free ethanol-water (EW) pretreatment with low ethanol concentration was developed. With less cellulose loss and similar hemicellulose removal, adding 10% (v/v) ethanol into water (EW10 pretreatment) resulted in a lignin removal...
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