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A surface plasmon resonance (SPR) assay for the specific recognition of a single guanine (G) bulge in DNA using an SPR sensor with an immobilized small-molecule ligand was developed. The synthesized ligand, 2-(2-aminoacetyl)amino-5,6,7-trimethyl-1,8 naphthyridine, can selectively binds to a G bulge of duplex DNA (dsDNA) through hydrogen bonding. The melting temperature (T m ) of the duplex...
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