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The effects of recombinant human growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were investigated in burned rats. Sprague-Dawley rats were fed exclusively by total parenteral nutrition and were subjected to 20% third-degree scald burns. The rats were then divided into the following three groups: (1) the GH group received GH at a dose of 1 IU kg -1 d -1 ...
The clinical significance of lipoprotein-X (Lp-X) induced by intravenous infusion of 10% fat emulsion was assessed, with special reference to atherogenesis, by in vitro experiment using purified Lp-X from the sera of patients receiving Intralipid 10%. Lp-X appeared after long-term intravenous infusion of 10% fat emulsion in the patients with intestinal fistula due to the anastomotic leakage. To...
Protein metabolism of skeletal muscle was studied in relation to severity of surgical stress and whole body protein metabolism in 24 patients undergoing a major surgical operation.The contribution of skeletal muscle protein breakdown to whole body protein breakdown increased significantly after surgical operation. There was a positive correlation between the contribution and urinary catecholamine...
The purpose of this study was to investigate the effects of human growth hormone (GH) on gene expression of albumin in the liver of burned rats.Eight male Sprague-Dawley rats (8 weeks old) were fed exclusively by TPN (200 kcal/kg/day, 6.2 g amino acid/kg/day) for 5 days. Then they were subjected to 20% full-thickness scald burns, and were divided into two groups. One group received 200 mU/day GH...
The purpose of this study was to investigate the effects of growth hormone (GH) on mRNA levels of structural proteins in skeletal muscle and diaphragm in burn injury.Eleven male Sprague-Dawley rats (7 weeks old) were fed exclusively by TPN for 5 days. Then they were subjected to 20% full-thickness scald burns, and were divided into two groups. One group received 200 mU/day of GH (GH group), and...
A study was conducted to clarify the quantitative relationship between the alteration of protein metabolism and the severity of surgical stress to further understand the mechanisms of body nitrogen losses in surgical trauma. Twenty-one patients undergoing esophagectomy for esophageal cancer (group E), and 22 undergoing gastrectomy or colorectal operations for gastric or colorectal cancer (Group GC)...
A study was conducted to clarify the contribution by myofibrillar protein to whole-body protein breakdown in surgically stressed patients. Thirteen patients who underwent esophagectomy (group E) and 22 who underwent gastric or colorectal operation (group GC) were studied. Patients were all male and younger than 65 y old. Whole-body protein breakdown was determined using constant infusion of ...
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