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We previously reported that the maize pollen-specific gene Zm401 functions in anther development. In this study, a Zm401’s ortholog Si401 was amplified and cloned from foxtail millet (Setaria italica). Zm401 and Si401 are highly conserved with 99% of their coding sequences identical to each other. Southern blot and RT-PCR analysis reveal that Si401 has only one copy in the genome and is expressed...
Summary Little work has been reported on genetic transformation with maize inbred lines, especially elite inbred lines used in breeding. In this work, 7 self-pollinated inbred lines and 4 hybrid lines have been screened. The results revealed that calli derived from immature embryos from two inbred lines X333 and X301 were compact, hyperhydric and unsuitable for transformation, but the calli induced...
Our previous study shows that the maize zm401 cDNA is specifically expressed in maize pollens. This evidence suggests that zm401 likely functions in pollen growth and/or development. To confirm its possible involvement in pollen development, the full length cDNA of zm40l was ectopically expressed in tobacco plants under the control of a pollen specific promoter ZM13 (from maize). RT-PCR amplification...
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