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Nonlinear optical microscopy (NLOM) was applied for monitoring dermal wound healing after mesenchymal stem cells (MSCs) transplantation. Our results showed that NLOM can reveal different regeneration-mechanisms of collagen in non-treated and MSCs-treated wound dermis.
We extend multiphoton microscopy to study the collagen remodeling and transplanted autologous fibroblasts metabolic states in mouse dermis after cellular injection.
The changes of collagen in sclerodermatous skin were investigated qualitatively and quantitatively using second harmonic generation (SHG) microscopy. The technique has great potential to be used to diagnose scleroderma and monitor the therapy response.
In dermis, collagen and elastin are important structural proteins of extracellular maxtrix. The matrix-disorder is associated with various physiologic processes, such as localized scleroderma, anetoderma, photoaging. In this work, we demonstrate the capability of nonlinear optical microscopy in imaging structural proteins in normal and pathological human dermis.
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