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Mpox (previously known as Monkeypox) has recently re‐emerged, primarily through human‐to‐human transmission in non‐endemic countries including India. Virus isolation is still considered as the gold standard for diagnosis of viral infections. Here, the qPCR positive skin lesion sample from a patient was inoculated in Vero E6 cell monolayer. Characteristic cytopathic effect exhibiting typical cell rounding...