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A simple, rapid, and selective ultra‐performance liquid chromatography–tandem mass spectrometry method for determination of l‐carnitine (LC) and acetyl‐l‐carnitine (ALC) in human serum was developed. Acetyl‐l‐carnitine‐d3 (ALC‐d3) was selected as internal standard (IS). After protein precipitation with acetonitrile–water (1 mL, 2:1, v/v), the analytes and IS were separated on a 2.5‐μm XSelect HSS...
A sensitive and selective high‐performance liquid chromatography–tandam mass spectrometry (LC–MS/MS) method was developed and validated for the simultaneous quantification of sildenafil and its metabolite N‐desmethyl sildenafil in human plasma. Sildenafil‐d8 was used as an internal standard. The analytes were extracted by precipitation extraction and chromatographed on a C18 column using mobile phase...
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