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The full-length cDNA of GDP-fucose transporter (GFT) was cloned from Chinese hamster ovary (CHO) 13D–35D cells. The predicted product consisted of 366 amino acids with 86% and 92% similarities to human GFT and mouse GFT, respectively. When synthetic small interfering RNA (siRNA) against CHO GFT was introduced into human antithrombin III (AT-III)-producing recombinant CHO cells, defucosylated AT-III...
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