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We propose a method of constructing three-dimensional (3D) biological microstructures by a pick-and-place micro-assembly of 2D micro-parts where living cells are loaded. The method includes four elemental techniques: (i) 2D cell-laden micro-plates, (ii) support structures for 3D assembly, (iii) pick-and-place micromanipulation system, (iv) biocompatible micro-gluing in aqueous environments. The advantage...
This paper describes a microfluidic device for a high through-put ion channel recordings, in which planar bilayer lipid membranes (BLMs) are spanning across a nanometer-sized poly(p-xylylene) (parylene) pore (Figure 1). To make the nanopore, a micrometer-sized pore of parylene was prepared by photolithography, and then parylene was additionally deposited on to the micropore with the isotropic vaporization...
This paper describes a method to transplant neurospheroid network onto the rat brain. We first patterned the uniform sized neurospheroids on the PDMS microchamber, and cultured for 1-2 weeks. After 2-week culture, the neurospheroids tightly connected each other with extending their neuronal processes (e.g. dendrite, axons) and formed neuronal network. We realized that the neurospheroid network can...
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