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Extracellular polymeric substances (EPS) were extracted from sludge and biofilm via the completely autotrophic nitrogen removal over nitrite (CANON) system. Tightly bound (TB)-EPS were extracted using four physical methods, namely, cationic exchange resin (CER), sonication, heating, and steaming. CER was the most effective and most suitable method for extraction among the four methods. Moreover, the...
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