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This study was carried out to investigate the structural perturbation of the protein’s local structure by the denaturants under non-denaturing conditions. Crystal structure of CutA from an archaeon Pyrococcus horikosii (PhoCutA), a heavy-metal binding protein, was determined at 1.6-Å resolution in the presence of 3M guanidine HCl (GdnHCl). Native PhoCutA has a large number of short intramolecular...
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